Glyconeogenesis in Growing and Non-growing Cultures of Tetrahymena pyriformis

Replication and Functions of Macronuclear Dna in Synchronously Growing Populations of Tetrahymena Pyriformis

Antibacterial activity of synthetic dermaseptins against growing and non-growing Escherichia coli cultures.

We synthesized, and purified to homogeneity, four peptides with sequences corresponding to natural dermaseptins. We compared their in-vitro antibacterial potencies against growing and non-growing Escherichia coli cells under different oxygen conditions. The dermaseptins displayed different antibacterial potencies (MICs = 1-4 microM). Killing assays showed that slowly growing bacteria were more ...

Polysomal organization in growing and resting cultures and its relation to protein synthesis in Tetrahymena.

Cytosol from Tetrahymena cells growing at different rates was isolated and separated by centrifugation into polysomal and non-polysomal fractions. The RNAs of either fraction were separated chromatographically into poly(A)+ RNA and poly(A)-RNA. It was found that in resting cultures the total RNA per cell is only about half of that of rapidly growing cultures. All fractions of RNA were reduced p...

Deoxyribonucleic acid polymerase from Tetrahymena pyriformis. Purification and properties of the major activity in exponentially growing cells.

The major DNA polymerase activity from exponentially growing Tefrahymena pyriformis was isolated and purified approximately l&000-fold relative to the specific activity of DNA polymerase in crude extracts. The enzyme aggregates at low ionic strength. In solutions of ionic strength 0.25 M or greater, the enzyme exists as a single species with an approximate molecular weight of 80,000. DNase acti...

Nonidentity of ribosomal structural proteins in growing and starved Tetrahymena

We have examined the ribosomal structural proteins isolated from vegetatively growing Tetrahymena pyriformis and from cells that had been starved of all nutrients for 24 h. Reproducible, nonartifactual differences in protein complement, primarily associated with the large ribosomal subunit, were found. The kinetics of change in ribosomal protein complement were followed both in refed and in new...